Transcriptomic analysis at 48 h postmortem: a proof of concept for the identification of biomarkers to estimate time since death.

ABSTRACT:

Background

The postmortem interval (PMI) refers to the time elapsed between an individual's death and the examination of the body. Tissues undergo a sequence of anatomical changes following death, which are routinely used to estimate the PMI.

Methods

To determine if these anatomical changes are associated with identifiable genomic adaptations that could characterize the PMI more accurately, we analyzed the rat skeletal muscle transcriptome at 0 and 48 h postmortem using Clariom™ S arrays. This study investigates whether specific transcriptomic changes correlate with PMI progression, offering a potential molecular tool to complement established anatomical methods.

Results

A total of 3,873 differentially expressed mRNAs were identified, of which 2,787 downregulated and

SUBMITTER: Zepeta Flores N 

PROVIDER: S-EPMC12540568 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature