Optimizing rhamnolipid bio-surfactant production in a bioreactor using waste glycerol.

ABSTRACT: Rhamnolipids (RLs) are glycolipid bio-surfactants produced by microorganisms with applications in industries, including environmental remediation and oil recovery, comparable to chemical surfactants. However, the reproducibility and scalability of RLs production in shake flask systems limit their industrial use, prompting the need for advanced bioreactor systems. This study aims to address this challenge by optimizing RLs production by Pseudomonas aeruginosa RS6 using treated waste glycerol (TWG), a low-cost by-product of biodiesel production, as a carbon source. Response surface methodology (RSM) was employed to evaluate the combined impact of TWG concentration, aeration, and agitation rates on RLs production and microbial behavior within a bioreactor system. Optimal conditions were then

SUBMITTER: Mahamad SS 

PROVIDER: S-EPMC12540569 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature