In vitro study on reversible regulation of CAR-T function by Kinsenoside.

ABSTRACT:

Backgrounds

Chimeric antigen receptor T-cell (CAR-T) therapy has effectively treated various types of malignant tumors. However, effective methods to address its excessive expansion are currently lacking.

Aims

We aimed to identify a natural compound that can safely and reversibly regulate CAR-T cell function.

Materials and methods

This study combined virtual screening of network pharmacology with in vitro experimental verification to evaluate how a natural small-molecule kinsenoside (KD) can dynamically regulate the function of CAR-T cells. Human and CAR-T cells were treated with KD. Following the intervention and subsequent drug withdrawal, we conducted flow cytometry for T-cell functional tests, such as cell proliferation, cell cycle, and cytotoxicity. Additionally,

SUBMITTER: Li G 

PROVIDER: S-EPMC12540606 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature