Central composite design optimized fluorescent method using dual doped graphene quantum dots for lacosamide determination in biological samples.

ABSTRACT: Lacosamide is a third-generation antiepileptic drug used for the treatment of partial-onset seizures. This study investigates the development and validation of a novel analytical method for the determination of lacosamide in human plasma and pharmaceutical formulations using boron and nitrogen co-doped graphene quantum dots (BN-GQDs). The prepared BN-GQDs exhibited excellent fluorescence properties that quenches in the presence of lacosamide, allowing for its sensitive detection. Density functional theory calculations and Stern-Volmer analysis were used to elucidate the mechanism of this fluorescence quenching, which was influenced by various factors optimized using a central composite design. The proposed method was validated according to ICH M10 guidelines displaying excellent linearity

SUBMITTER: Serag A 

PROVIDER: S-EPMC12540684 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature