Mapping the BCSC-1 interactome in breast cancer.

ABSTRACT: BCSC-1 (breast cancer suppressor candidate-1), the official gene symbol VWA5A (von Willebrand factor A domain containing 5 A), is downregulated in a variety of cancer types including breast cancer. The role of BCSC-1 in tumorigenesis and the underlying mechanisms are poorly understood. One of the ways to predict a spectrum of functions of an unknown protein is to identify the protein-protein interaction network involving it. Flag-pLV-Neo-BCSC1 or the empty vector were stably transfected into breast cancer cells MCF-7. The levels of BCSC1 expression were identified by Western blotting. Co-immunoprecipitation assay coupled with liquid chromatography with tandem mass spectrometry (Co-IP-MS) and bioinformatic analysis were done to study protein-protein interactions. A subset of interacting pro

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12540739 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature