A Bayesian approach towards atomically-precise localization in fluorescence microscopy.

ABSTRACT: Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy, have pushed localization below nanometer precision, by applying prior knowledge of correlated fluorescence emission from single emitters. However, achieving a refinement from 1 nm to 1 Ångström demands a hundred-fold increase in collected photon signal. This quadratic resource scaling imposes a fundamental barrier in single-molecule localization microscopy, where the intense photon collection is challenged by photo-bleaching, prolonged integration times, and inherent practical constraints. Here, we break this limit by harnessing the periodic nature of the atomic lattice s

SUBMITTER: Duan Y 

PROVIDER: S-EPMC12541000 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature