The genetically encoded biosensor FEOX is a molecular gauge for cellular iron environment dynamics at single cell resolution.

ABSTRACT: Iron-sensing is evolutionarily conserved among life on Earth, and mammalian cells are known to sense cellular iron through mechanisms similar to bacteria and marine invertebrates. While iron regulatory proteins (IRPs) function as RNA-binding proteins during iron-limiting conditions and reflect cellular iron status, we lack genetically-encodeable tools to quantify bioavailable intracellular iron status over developmental time and at single-cell resolution. In order to better understand the cellular environment that supports or restricts IRP-active conditions, particularly during complex and dynamic changes leading to differentiated states, we present a ratiometric genetically-encoded biosensor called FEOX. FEOX is a ratiometric fluorescent biosensor of the cellular iron environment based on

SUBMITTER: Sangokoya C 

PROVIDER: S-EPMC12541023 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature