Diversity of oxidative stress and senescence phenotypes induced by chemotherapeutic agents in HUVECs.

ABSTRACT: Chemotherapy-induced endothelial cell senescence impacts tumor angiogenesis and therapy outcomes. While senescence can suppress tumorigenesis, it may also promote chronic inflammation and vascular toxicity. This study investigates how different chemotherapeutic agents induce senescence in human umbilical vein endothelial cells (HUVECs) and explores the roles of reactive oxygen species (ROS) and ataxia telangiectasia mutated (ATM) kinase. HUVECs were treated with DNA crosslinkers (doxorubicin, mitomycin C), topoisomerase inhibitors (etoposide, camptothecin), and methotrexate (MTX). Senescence was assessed via senescence-associated β-galactosidase (SA-β-gal) staining, senescence-associated secretory phenotype related factors, Phalloidin staining, immunofluorescence (p53, Ki67, 53BP1, γH2AX),

SUBMITTER: Zhong X 

PROVIDER: S-EPMC12541024 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature