Transpupillary in vivo two-photon imaging reveals enhanced surveillance of retinal microglia in diabetic mice.

ABSTRACT: The retina, located outside the cranium, serves as an ideal structure for investigating information processing within the central nervous system due to its well-organized neurovascular unit comprising diverse cell types, including neurons, glial cells (such as microglia, Müller glia, and astrocytes), pericytes, and vascular endothelial cells. Disrupted retinal homeostasis contributes to various ocular diseases such as diabetic retinopathy, age-related macular degeneration, glaucoma, and retinitis pigmentosa. However, noninvasive in vivo imaging methods to study the pathogenesis of these disorders remain limited. Here, we developed a two-photon microscopy technique for real-time, transpupillary in vivo visualization of the retinal neurovascular unit in mice. Our approach integrates systemat

SUBMITTER: Sotani N 

PROVIDER: S-EPMC12541322 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature