Ischaemic preconditioning regulates cardiac transcriptome via DNA methylation conferring cardio-protection from ischaemic reperfusion injury.

ABSTRACT:

Aims

Myocardial ischaemic preconditioning (IPC) increases myocardial ability to withstand ischaemic injury. Myocardial stunning is a reversible dysfunction, while necrosis results in irreversible cell death. The link between IPC, stunning, and necrosis remains unclear. This study aimed to utilize a novel 13.5-min ischaemia-reperfusion (I/R) rat model, distinct from conventional I/R models, to identify transcriptomic changes associated with IPC and investigate the role of DNA methylation in regulating these changes, particularly in relation to myocardial stunning and necrosis.

Methods and results

A novel rat model of cardiac I/R injury was used, with IPC induced by two 5-min ischaemia-reperfusion cycles followed by 13.5-min of ischaemia, and a control group undergoing 13.5-mi

SUBMITTER: Khan AW 

PROVIDER: S-EPMC12541389 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature