Tumor mutational burden, gene expression patterns, and immune-cell deconvolution in testicular germ cell tumors.

ABSTRACT:

Background

Testicular germ cell tumor (TGCT) is the most common malignancy in young males from ages 15 to 44 years, but treatment remains challenging. Emerging evidence indicates that the tumor mutational burden (TMB) may be associated with tumor growth and progression. This study examined the association of TMB with the gene expression profiles and the tumor microenvironment (TME) of TGCT.

Methods

Datasets from the Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) databases were analyzed. Differential expression analysis was applied to identify TMB-related genes via DESeq2 in TCGA. Further protein-protein interaction (PPI) network analysis, including module and degree analysis combined with Kaplan-Meier analysis, was applie

SUBMITTER: Wang L 

PROVIDER: S-EPMC12541522 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature