In Vivo Online Monitoring of Intracellular Lipid Accumulation in <i>Ustilago maydis</i>.

ABSTRACT: Single cell oils produced in microorganisms constitute appealing alternatives to plant oils. Oleaginous fungi accumulate triacylglycerols in lipid droplets (LD). Their biosynthesis is typically induced under nitrogen limitation. We exploit the fungal model Ustilago maydis for oil production. The stain 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) can be used to track LD formation during cultivation but this expensive compound is only affordable at small-scale. Therefore, mutant screening for optimization of oil production and composition would benefit from an inexpensive online monitoring system. Accordingly, we aimed at developing an intrinsic reporter that is suitable to track oil formation even in larger cultures. From three tested candidates, the pote

SUBMITTER: Muntjes K 

PROVIDER: S-EPMC12541552 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature