Production of the Antimicrobial Roseoflavin With Genetically Engineered Corynebacterium glutamicum.

ABSTRACT: The global rise of drug-resistant bacterial infections underscores an urgent demand for novel antimicrobial agents. Roseoflavin, a naturally occurring riboflavin analog, has emerged as a promising candidate for drug development. In this study, the industrial workhorse Corynebacterium glutamicum was metabolically engineered towards roseoflavin production. Overexpression of the roseoflavin biosynthetic genes rosABC and the flavin transport gene ribM from the native roseoflavin producer Streptomyces davaonensis was evaluated in C. glutamicum. To further link roseoflavin biosynthesis to its riboflavin precursor, the riboflavin kinase gene ribF from both S. davaonensis and C. glutamicum was evaluated, revealing that its overexpression is essential for enhancing roseoflavin production. The final

SUBMITTER: Brito LF 

PROVIDER: S-EPMC12541554 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature