Identification of MscS as a Key L-Glutamate Exporter in Bacillus methanolicus.

ABSTRACT: Small-conductance mechanosensitive channels (MscS) are established l-glutamate exporters in industrially relevant bacteria, yet their role in the methylotrophic bacterium Bacillus methanolicus, a promising platform for sustainable methanol-based l-glutamate production, remains unexplored. Our research on B. methanolicus MGA3 identifies its MscS as the sole mechanosensitive channel in this organism and a key exporter of the amino acid l-glutamate, providing valuable insights into its potential for industrial applications. Transcriptomic analysis of B. methanolicus wild type cultured on an l-glutamate production medium revealed downregulation of the fatty acid biosynthesis genes fadR, fadF, mutB2, and acdA, suggesting that fatty acid metabolism is influenced by l-glutamate overproduction, wi

SUBMITTER: Brito LF 

PROVIDER: S-EPMC12541555 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature