Stability and Pseudocatecholase Activity of Artificial Bis-Histidyl Copper Peptides.

ABSTRACT: In this paper, 4-methylcatechol (4-MC) and L/D-Dopa are selected as target substrates for de novo protein design, serving as a proof-of-concept system for evaluating the catalytic activity of metal coordination sites. We report two novel water-soluble bis-histidyl peptides that exhibit catalytic activity, where structural constraints around the active site would play a pivotal role in metalloenzyme development. The particular tandem His-His motif in the peptides and favorable E₀' enable them to catalyze catechol oxidation reactions efficiently. The coordination behavior of the peptides with Cu(II) and Cu(I) ions is thoroughly investigated using a combination of analytical techniques, including potentiometric titration and fluorescence, ultraviolet-visible (UV-vis),

SUBMITTER: Bottoni C 

PROVIDER: S-EPMC12541693 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature