Comparative aptamer profiling reveals cell surface remodeling and the emergence of a noncanonical cell surface protein under oncogenic signaling.

ABSTRACT: Identifying cell type-specific molecular markers on the cancer cell surface is essential for understanding cancer progression and for discovering critical neoantigens relevant to immunotherapy. Nucleic acid aptamers serve as powerful tools for probing complex and dynamic cell surface characteristics. Here, we introduce a streamlined comparative aptamer profiling methodology that enables side-by-side analysis of cell surface remodeling. Using cell-SELEX (systematic evolution of ligands by exponential enrichment), we generated a modified base-incorporated aptamer library directly from cells, which was then employed to explore the surface states of normal and mutant protein-expressing cells. Differential analysis of aptamer enrichment using next-generation sequencing revealed distinct aptamer

SUBMITTER: Kakuta J 

PROVIDER: S-EPMC12541808 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature