Single-cell and spatial analysis reveals macrophage-T cell crosstalk in non-small cell lung cancer immunosuppression.

ABSTRACT:

Background

The tumor microenvironment (TME) is instrumental in facilitating immune evasion and promoting tumor progression in non-small cell lung cancer (NSCLC). However, the spatial heterogeneity and molecular interactions between immune and non-immune cells are not yet fully understood. This research amalgamated single-cell RNA sequencing (scRNA-seq) and spatial transcriptomic data to examine the cellular composition, ligand-receptor (LR) interactions, and immunosuppressive mechanisms of NSCLC.

Methods

ScRNA-seq was performed on NSCLC tumors and paired adjacent normal tissues to analyze immune and stromal cell heterogeneity. Spatial transcriptomics was applied to consecutive tissue sections to map the distribution of major cell types and their interactions. Cell clustering

SUBMITTER: Zhu P 

PROVIDER: S-EPMC12541844 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature