Challenges in mimicking hypoxia: insights into HIF-regulated MiRNA expression induced by DMOG and CoCl₂.

ABSTRACT: The cellular and tissue response to acute hypoxia is mediated by a complex adaptive response initiated by hypoxia-inducible factors (HIF) 1 and 2. HIF proteins are key transcription factors that modulate gene expression to adapt cells to the low oxygen tension. Cellular studies of hypoxia have used the low oxygen model by using hypoxia chambers to monitor the gene expression changes, while others have utilized hypoxia mimetics in normoxic conditions. Here, we provide a comparison of the utility and limitations of two hypoxia mimetics by studying the changes in the miRNA and mRNA expression profiles compared to the low oxygen model. We utilized data from NGS-based global analyses, qPCR validation, and HIFa silencing experiments to determine the distinct profiles of HIF-1 and HIF-2 activitie

SUBMITTER: Barton A 

PROVIDER: S-EPMC12542114 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature