Integrative single-cell analysis of metabolic syndrome reveals novel cellular heterogeneity and differentiation dynamics in adipose tissue.

ABSTRACT:

Background

Metabolic syndrome (MetS) is characterized by obesity, insulin resistance, and dyslipidemia with adipose tissue inflammation, yet its cellular heterogeneity and intercellular interactions remain poorly understood. We analyzed single-nucleus RNA sequencing data from subcutaneous adipose tissue of 84 individuals with MetS from the METSIM cohort, characterizing cell composition, inter-individual variation, adipocyte progenitor differentiation, and cell-cell communication networks.

Methods

We performed single-nucleus RNA sequencing on subcutaneous adipose tissue samples from 84 individuals with MetS. Clustering analysis was used to define cell types and subpopulations, inter-individual variation in cell composition was assessed, pseudotime trajectory analysis reconstr

SUBMITTER: Jia H 

PROVIDER: S-EPMC12542196 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature