Ex vivo modelling of human colorectal cancer liver metastasis by normothermic machine perfusion.

ABSTRACT:

Background

Colorectal cancer liver metastasis (CRLM) is associated with poor survival, primarily due to acquired therapy resistance. While novel therapies arise, translation is limited by the lack of tumor models accurately representing dynamic microenvironmental interplay. Here, we show that ex vivo normothermic machine perfusion (NMP) offers a novel preclinical framework to study the intratumoral dynamics of CRLM biology.

Methods

Six resected metastatic human livers were preserved for two days and subjected to multi-omic profiling of serially sampled adjacent liver and metastatic tissue using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST). Tissue integrity was assessed and cross-validated by immunofluorescence (IF), high-resolution respirometry (HR

SUBMITTER: Trebo M 

PROVIDER: S-EPMC12542284 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature