Phage vB_KpnM_JYSS3 encodes a novel polysaccharide depolymerase that exhibits specific activity against K2-type carbapenem-resistant Klebsiella pneumoniae.

ABSTRACT:

Background

Carbapenem-resistant Klebsiella pneumoniae (CRKP), a major nosocomial pathogen, poses an increasingly serious threat to human health.

Results

In the quest for innovative therapeutic modalities to treat CRKP infections, a newly discovered phage, vB_KpnM_JYSS3, which can specifically infect and lyse K2-type CRKP, was isolated and subjected to an array of comprehensive characterizations and genome sequencing. Furthermore, an analysis of the expression of the tail protein (ORF17) and the detection of its functions were carried out. Double-layer agar plates containing CRKP were used to isolate phages from hospital sewage samples. The phage's optimal multiplicity of infection (MOI), lytic spectrum, bactericidal activity, stability, and one-step growth curve were tested.

SUBMITTER: Jiao SY 

PROVIDER: S-EPMC12542494 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature