A systems-level insight into PHB-driven metabolic adaptation orchestrated by the PHB-binding transcriptional regulator AniA (PhaR).

ABSTRACT: Poly(3-hydroxybutyrate) (PHB) is a carbon and energy storage polymer, whose accumulation under nutrient imbalances with excess carbon is common in bacteria. PhaR is a conserved transcriptional regulator that associates with PHB granules in several species. Although its role in modulating PHB storage and metabolism has been extensively studied across the bacterial phylogeny, a systems-level view of PhaR's dual function as a metabolic sensor and regulator is lacking. Here, we integrated co-expression network analysis with proteome profiling across multiple mutant backgrounds (lack of PhaR [AniA] and/or PHB synthesis) in the free-living state of the PHB-accumulating α-proteobacterial root nodule symbiont Sinorhizobium meliloti. This analysis was enriched by identifying direct regulator

SUBMITTER: Lagares A 

PROVIDER: S-EPMC12542648 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature