Metagenomic research on the structural difference of plaque microbiome from different caries stages and the construction of a caries diagnostic model.

ABSTRACT: Development of dental caries is a dynamic process; yet, there is limited knowledge on microbial differences at various stages of caries at higher resolution. To investigate the shifting microbiome profiles across different caries stages, 30 children were enrolled in this study, including 15 caries-active patients and 15 caries-free individuals. Plaque samples were collected from the buccal surface of caries-free subjects, defined as confident health (CH; n = 15). For caries-active individuals, plaque samples were collected from non-cavitated surfaces (defined as relative health [RH], n = 15), enamel caries (EC; n = 15), and dentin caries samples (DC; n = 15). All the above samples were sequenced through the 2bRAD sequencing platform to reveal the microbial commu

SUBMITTER: Dong L 

PROVIDER: S-EPMC12542677 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature