Establishment of an antimetabolite-based transformation system for the wood-decaying basidiomycete <i>Phanerochaete chrysosporium</i>.

ABSTRACT: The model wood-decaying basidiomycete Phanerochaete chrysosporium has been extensively studied to elucidate the molecular mechanisms of wood decomposition. However, genetic studies have been limited by the lack of adequate genetic tools. Here, we established an antimetabolite-based transformation system, originally developed for ascomycetes, for use in P. chrysosporium. The transformation system utilizes pyrithiamine (PT), a thiamine antimetabolite, in combination with the pPTRII vector that contains the PT resistance gene (ptrA). PT effectively inhibited the growth of P. chrysosporium, and the introduction of ptrA conferred resistance to transformant mycelia. The transformation efficiency was comparable to that in ascomycetes, suggesting that the transfo

SUBMITTER: Masumoto K 

PROVIDER: S-EPMC12542683 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature