Recovering wastewater RNA for virome sequencing by systematically optimized tangential-flow ultrafiltration and Nanotrap microbiome particles.

ABSTRACT: Municipal wastewater harbors diverse RNA viruses, which are responsible for many emerging and reemerging diseases in humans, animals, and plants. Although genomic sequencing can be a high-throughput approach for profiling the RNA virome in wastewater, wastewater processing methods often influence sequencing outcomes. Here, we systematically evaluated two wastewater processing methods, tangential-flow ultrafiltration (TFF) and Nanotrap Microbiome A Particles, for detecting the target RNA virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via amplicon sequencing and characterizing the RNA virome using whole-transcriptome shotgun sequencing. Our results from paired comparison tests showed that the TFF and Nanotrap methods recovered similar SARS-CoV-2 variants at the lineage le

SUBMITTER: Segelhurst E 

PROVIDER: S-EPMC12542684 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature