Characterization of isolates used in bacterial-based strategies for accelerated carbonation of lime mortars.

ABSTRACT: Portland cement largely replaced hydraulic lime over the past century because of its rapid hardening. Achieving earlier hardening in lime through faster carbonation is thus essential to help overcome one of lime's limiting qualities. Here, we isolated two alkaliphilic bacteria, Shouchella clausii and Shouchella patagoniensis, from a lime mortar wall. S. clausii was then further grown in high pH (>11) by adaptive laboratory evolution to acclimate a third isolate. Bacterial suspensions of all three isolates were followed for 14 days in serum bottles at pH 11, and gas composition of the headspace, intact/damaged cell populations, and pH were measured. In parallel, lime mortar samples were incubated in a closed environment with bacterial suspension of the isolates and anal

SUBMITTER: Grosso Giordano F 

PROVIDER: S-EPMC12542689 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature