A three-plasmid-containing CRISPR-Cas9 platform to engineer <i>Bacillus velezensis</i> 916 as an efficient biocontrol agent.

ABSTRACT: Bacillus velezensis (Bv) is a widely used biocontrol agent against plant diseases, mainly because its genome contains numerous non-ribosomal peptide synthetases (NRPS) gene clusters for the synthesis of various cyclic lipopeptides (CLPs). The domesticated strain Bv916, capable of co-producing four CLPs, has been successfully applied for green control of rice sheath blight and angular leaf spot. To enhance Bv916's biological control efficacy while maintaining environmental safety, it is essential to establish a food-grade gene editing platform in Bv916. Here, a three-plasmid CRISPR-Cas9 platform for Bv916 was constructed using the thermosensitive origin pET194ts, constitutive P43 promoters for Cas9, the specific promoter Psrf for single guide RNAs (sgRNAs), and three resistance gene

SUBMITTER: Li L 

PROVIDER: S-EPMC12542745 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature