Autophagy related 14 protects against liver injury by inhibiting multiple cell death pathways.

ABSTRACT:

Background

Autophagy is critical for cellular homeostasis. Autophagy related 14 (ATG14) is a key regulator of autophagy initiation; however, its role in hepatocyte survival remains poorly understood. This study aimed to investigate the hepatocyte-specific function of ATG14 in vivo.

Methods

We generated Atg14 hepatocyte-specific knockout (HepKO) mice using adeno-associated virus to deliver thyroxine-binding globulin gene promoter-driven Cre into Atg14 floxed mice at an adult age and fed control and knockout mice with either normal chow or a Western diet. Blood and tissue samples were collected for biochemical and histological analyses.

Results

Atg14 HepKO mice develop severe hepatomegaly under normal dietary conditions. ATG14 deficiency lead

SUBMITTER: Kim HG 

PROVIDER: S-EPMC12542747 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature