A thermostable Cas9-based genome editing system for thermophilic acetogenic bacterium <i>Thermoanaerobacter kivui</i>.

ABSTRACT: Thermoanaerobacter kivui is a thermophilic acetogenic bacterium capable of thriving at elevated temperatures up to 66°C. It metabolizes carbohydrates such as glucose, mannose, and fructose and can also grow lithotrophically utilizing hydrogen (H₂) and carbon dioxide (CO₂) or carbon monoxide (CO), with acetate serving as its main product. A simple and efficient genome editing system for T. kivui would not only facilitate the understanding of the physiological function of enzymes involved in energy and carbon metabolism but also enable metabolic engineering. To address this issue, we developed a thermostable Cas9-based genome editing system for targeted gene knockout and gene integration into the T. kivui genome. Gene knockout assays were conducted

SUBMITTER: Le Y 

PROVIDER: S-EPMC12542768 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature