Activatable fluorescent ratiometric probes for early diagnosis and prognostic assessment of acute kidney injury.

ABSTRACT: Early and accurate diagnosis of acute kidney injury (AKI) is crucial for clinical treatment. However, most existing fluorescent probes are prone to background interference and limited renal targeting. We developed a ratiometric nanoprobe (RP-SC) that synergistically responded to H₂O₂ and targeted kidney injury molecule-1 (KIM-1). In AKI kidneys, RP-SC was hydrolyzed and slowly released encapsulated Hcy-BOH and Cy-Dopa. The H₂O₂ level was semi-quantitatively analyzed by fluorescence ratio (F_Hcy-BOH/F_Cy-Dopa). RP-SC exhibited prominent renal fluorescence in AKI mice, making it possible to accurately diagnose AKI and dynamically monitor renal function for up to 60 hours. RP-SC could monitor the process of AKI in v

SUBMITTER: Li N 

PROVIDER: S-EPMC12542964 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature