Behavioural, immunological and transcriptomic consequences of post-weaning social isolation and chronic celecoxib administration in mouse.

ABSTRACT: Early life stress (ELS) and chronic low-grade inflammation are associated with psychiatric disease risk, but their neurobiological consequences are poorly understood. Here, we aim to investigate the behavioural, immunological and molecular consequences of ELS in mice. C57Bl6 mice were subjected to post-weaning social isolation (SI - PD21-40) with or without chronic celecoxib (CEL) (PD21-61). ELS-induced behavioural changes were assessed using the open field test (OFT) and three-chambered test (3CT). The anti-inflammatory effects of celecoxib were assessed by enzyme-linked immunosorbent assay (ELISA) of IL-6, TNF-α and IL-10 cytokines released by stimulated splenocytes. Gene expression changes in the hippocampus and amygdala were assessed using RNA-sequencing. Neither SI nor CEL affected OF

SUBMITTER: Laighneach A 

PROVIDER: S-EPMC12543112 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature