Diagnosis of Trypanosoma cruzi infection in Mexican populations: current conventional serology lacks adequate sensitivity and specificity.

ABSTRACT:

Background

The performance of serological tests for Trypanosoma cruzi diagnosis in Mexico has not included discordant control sera nor has it evaluated the role of immune response specificities, patient infection history or clinical status.

Objectives

The performance of commercial serological and molecular diagnostic tests and diagnostic algorithms was analysed in Mixtecan and Zapotecan ethnic populations having recent and long-term infection history.

Methods

An amplified global gold standard for T. cruzi infection included serological (≥ 2 conventional tests positive) and molecular (sequence identity of any of five genes using end point polymerase chain reaction (epPCR) or any positive using quantitative polymerase chain reaction (qPCR) diagnostic test results.

Fi

SUBMITTER: Ramsey JM 

PROVIDER: S-EPMC12543361 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature