CD9+ and CD82+ extracellular vesicles in synovial fluid differentiate aseptic from septic endoprosthesis loosening.

ABSTRACT: Aim: Extracellular vesicles (EVs) hold great promise as emerging biomarkers for a variety of diseases. However, their clinical application is still hindered by complex and time-consuming isolation procedures. A clinically relevant scenario where improved biomarker-based diagnostics are urgently needed is prosthetic joint loosening, which may result from either aseptic inflammation or periprosthetic joint infection (PJI) - two conditions requiring fundamentally different therapeutic approaches. This study investigated whether imaging flow cytometry (IFCM) enables the discrimination between aseptic and septic loosening by profiling EVs directly in minimally processed synovial fluid. Methods: We analyzed synovial fluid from 35 aseptic and 13 septic cases using IFCM to detect sur

SUBMITTER: Tertel T 

PROVIDER: S-EPMC12544082 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature