Constructing chimeric mouse islets to study alpha- and delta-cell influence on beta-cell feature.

ABSTRACT:

Objectives

This study aimed to evaluate the role of alpha- and delta-cell signals on beta-cells within pancreatic mouse islets. Specifically, we investigated how these signals regulate glucose sensitivity, gene expression and function in beta-cells.

Methods

We first implemented our previous protocol to FACS purify alpha-, beta-, and delta-cells by adding CD81 as a positive marker for alpha-cells. We next developed an approach to reaggregate these sorted cell populations, creating chimeric islets with different proportions of each endocrine cell type. We used these chimeric islets to study the effect of alpha- and delta-cells on glucose sensitivity, gene expression and function in beta-cells.

Results

We generated chimeric islets containing either all three endocrine ce

SUBMITTER: Fouque A 

PROVIDER: S-EPMC12544172 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature