SUMOylation of ZEB1 Modulates PANoptosis in Burn-Induced Early Acute Kidney Injury.

ABSTRACT: Burn-induced acute kidney injury (AKI) involves inflammatory programmed cell death pathways, including PANoptosis. This study investigated how SUMOylation of the transcription factor ZEB1 regulates PANoptosis in early burn-induced AKI. Human AKI datasets were analyzed for ZEB1 expression and correlation with PANoptosis/SUMOylation genes. A rat burn-AKI model (24-72 h) evaluated renal dysfunction (creatinine, urea), histopathology (H&E, TUNEL) and oxidative stress (MDA/SOD/CAT). In vitro, HK-2 cells under hypoxia/reoxygenation (H/R) assessed ZEB1 and PANoptosis effectors (Caspase-3/MLKL/GSDMD). Co-immunoprecipitation assessed ZEB1 SUMOylation and ubiquitination. Flow cytometry measured intracellular reactive oxygen species (ROS) levels. ELISA quantified IL-1β and IL-6. Functional studies us

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12544699 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature