Proteomic Analysis of Circulating IgA1-Containing Immune Complexes in Patients with IgA Nephropathy.

ABSTRACT:

Introduction

IgA1-containing immune complexes (IgA1-IC) consisting of galactose-deficient IgA1 (Gal-deficient IgA1) and IgG autoantibodies are central to the pathogenesis of IgA nephropathy (IgAN). These IgA1-IC form in circulation, and additional proteins may be added before the deposition in the glomeruli. However, the composition of these circulating IgA1-IC is not fully understood. To address this gap in knowledge, we developed a novel proteomic workflow.

Methods

IgA1-IC from sera of patients with IgAN and healthy controls (HCs) were isolated by lectin-affinity chromatography followed by size-exclusion chromatography (SEC). IgA1 yield and molecular integrity were assessed by enzyme-linked immunosorbent assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

SUBMITTER: Cunningham MA 

PROVIDER: S-EPMC12545556 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature