Exploring drug resistance genes in <i>Acinetobacter baumannii</i> using metagenomic next-generation sequencing.

ABSTRACT:

Introduction

With the rising incidence of infectious diseases, the overuse of antibiotics has become a serious problem in clinical practice. In recent years, metagenomic next-generation sequencing (mNGS) has emerged as a promising alternative, offering advantages such as rapid turnaround, broad-spectrum detection, and comprehensive coverage. However, its clinical utility for antimicrobial resistance testing remains to be fully established.

Methods

In this study, we evaluated the effectiveness of mNGS in detecting antimicrobial resistance in Acinetobacter baumannii using 53 clinical samples. The performance of mNGS was compared with conventional culture-based methods. In addition, clinical judgment of drug resistance was used as a reference to assess concordance between seque

SUBMITTER: Li G 

PROVIDER: S-EPMC12546095 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature