Transgene-free genome editing in citrus and poplar trees using positive and negative selection markers.

ABSTRACT:

Key message

Transgene-free genome editing of the gene of interest in citrus and poplar has been achieved by co-editing the ALS gene via transient transgene expression of an efficient cytosine base editor. CRISPR-Cas genome editing systems have been widely used in plants. However, such genome-edited plants are nearly always transgenic in the first generation when Agrobacterium-mediated transformation is used. Transgene-free genome-edited plants are valuable for genetic analysis and breeding as well as simplifying regulatory approval. It can be challenging to generate transgene-free genome-edited plants in vegetatively propagated or perennial plants. To advance transgene-free genome editing in citrus and poplar, we investigated a co-editing strategy using an efficient cytosine base e

SUBMITTER: Rocha DC 

PROVIDER: S-EPMC12546424 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature