Genistein enhances anti-PD-1 efficacy in melanoma by suppressing regulatory T cell differentiation and activity.

ABSTRACT: The accumulation of regulatory T cells (Tregs) in the tumor microenvironment is a key factor for immunosuppression and immune escape of cancer cells. Genistein is a natural isoflavonoid in soybeans, possessing a wide range of pharmacological bioactivities. However, the immunomodulatory potential of genistein remains largely unexplored. This study investigated the synergistic efficacy of genistein with anti-PD-1 therapy in a mouse model of melanoma. The regulatory role of genistein in Treg differentiation and functional stability was also examined in this study. The antitumor effects of genistein, alone or in combination with anti-PD-1 therapy, were evaluated in the mouse B16-F10 melanoma model. The profiles of intratumoral Tregs and CD8⁺ cytotoxic T cells were profiled using flo

SUBMITTER: Mo F 

PROVIDER: S-EPMC12546575 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature