Dax1 in AgRP neurons regulates thermogenesis via GR-HDAC3-mediated CRFR1 suppression.

ABSTRACT:

Objectives

The arcuate nucleus of the hypothalamus plays a pivotal role in metabolic homeostasis by integrating the functions of AgRP and POMC neurons. Dax1, a nuclear receptor-like transcription factor, is highly enriched in AgRP neurons; however, its role in energy balance regulation remains largely unexplored. Here, we demonstrate the function of Dax1 in hypothalamic AgRP neurons and its contribution to systemic energy homeostasis and thermogenesis in mice.

Methods

We generated AgRP neuron-specific Dax1 conditional knockout mice and assessed their physiological and metabolic responses under high-fat diet feeding and cold exposure. Energy expenditure, brown adipose tissue (BAT) thermogenesis, neuronal activation, and neuropeptide expression were evaluated. Molecular mechan

SUBMITTER: Wei Z 

PROVIDER: S-EPMC12546989 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature