The trypanosome vault particle is composed of multiple major vault protein paralogs and harbors vault RNA.

ABSTRACT: Many but not all Eukaryotes have protein-enclosed compartments called vaults. Vaults are composed of multiple copies of the major vault protein, symmetrically assembled into a basket-like shell. A human cell contains approximately 100,000 vault particles, the vast majority localized to the cytosol but also observed in the nucleus and at the nuclear pore complex. Whilst there is intriguing structural information of the vault shell, the function of vaults remains largely elusive, apart from a potential contribution to mRNA maturation. We set out to explore the vault interactome in the early branching eukaryote Trypanosoma brucei employing a combination of affinity capture and TurboID proximity labelling. T. brucei encodes three major vault protein (MVP) paralogs, which exhibit a considerable

SUBMITTER: Zavrelova A 

PROVIDER: S-EPMC12547018 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature