Neuronal activity-dependent gene expression is stimulus-specific and changes with neuronal maturation.

ABSTRACT:

Introduction

Neuronal activity-dependent gene expression is fundamental to a wide variety of brain functions. The field of neuronal activity-induced gene expression has advanced greatly due to studies performed in early neuronal cultures (7 to 10 DIV) and stimulated with different activation protocols. However, the effect of the developmental stage as well as the influence of specific protocol stimuli like potassium chloride (KCl)-induced depolarization, bicuculline (Bic)-mediated synaptic activation and TTX-withdrawal (TTXw) on activity-induced transcription has not been systematically studied.

Methods

To analyze the influence of neuronal maturation on activity-induced transcription, we used neuronal primary cultures to compare electrophysiological and transcriptional respo

SUBMITTER: Lukin J 

PROVIDER: S-EPMC12547137 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature