An Electrospun Heparin Modified Nanofiber Membrane Carrying Multiple Growth Factors for Dental Pulp Regeneration.

ABSTRACT:

Introduction and aims

To develop a multifunctional nanofiber membrane carrying angiogenic, dentinogenic, and neurogenic growth factors for dental pulp regeneration.

Methods

Electrospun gelatin/polycaprolactone (GEL/PCL) nanofiber membranes were modified with heparin (H-GEL/PCL) and loaded with VEGF, BMP-2, and NGF. The physicochemical properties and growth factor release behaviour of the membranes were systematically characterized. Their bioactivity was evaluated by assessing cell viability, proliferation, migration, and differentiation of human dental pulp stem cells in vitro, and a subcutaneous semiorthotopic transplantation model was established to verify the pulp-like tissue regeneration potential in vivo.

Results

The H-GEL/PCL membranes exhibited uniform nanofibe

SUBMITTER: Li XL 

PROVIDER: S-EPMC12547245 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature