Hierarchical clustering defines hypermethylated RSPO2 as early-stage potential biomarker in colorectal cancer.

ABSTRACT:

Background

Early-stage diagnosis, absence of specific reliable biomarkers, and better clinical management of colorectal cancer (CRC) remain major challenges. The Rspondin2 (RSPO2) gene is one of the most potent enablers of Wnt signaling, having contrasting aspects in tumor development and progression.

Aim

We aimed to identify the role of RSPO2 as a molecular biomarker by evaluating its methylation and expression profiles in CRC tissue samples to identify biomarker potential.

Methodology

Combined bisulfite restriction analysis (CoBRA) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to access the change in methylation pattern and expression profile of RSPO2 in 52 CRC tissue samples. DNA methylome and transcriptome profiling of

SUBMITTER: Srivastava A 

PROVIDER: S-EPMC12547467 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature