Genetically Encoded Cell Fate Reporter System for Multiplex Single-Cell Detection of DNA and Mitochondrial Damage.

ABSTRACT: Predicting the mechanism of action of bioactive compounds and toxicants is of great importance in drug discovery, as well as toxicology testing of environmental toxicants. Many reporter cell lines were created for generating phenotypic data in assigning the biological impact of large compound libraries. A major requirement of a phenotypic screen is the reporter cell lines for appropriate biomarkers with a good predictive value. Here, we have developed a stable cell expressing TagBFP2-53BP1 as a real-time sensor for DNA damage at the single-cell level that marks 53BP1 foci formation under physiological conditions, as well as toxicant-induced DNA damage. The cells were further engineered to report mitochondrial permeabilization using Smac-RFP, providing a broader application potential for th

SUBMITTER: Jayaprasad AG 

PROVIDER: S-EPMC12547572 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature