Biochemical characterization of phosphoenolpyruvate carboxykinases from multiple species of brown algae.

ABSTRACT: Phosphoenolpyruvate carboxykinase (PEPCK) is involved in the conversion of phosphoenolpyruvate (PEP) to oxaloacetate (OAA). In addition to playing a role in gluconeogenesis in various organisms, PEPCK also functions in the C₄ cycle to concentrate CO₂ for photosynthesis in some C₄ plants. Brown algae harbor genes related to the C₄ cycle, including the PEPCK gene, and are proposed to employ a C₄ cycle-like pathway. However, little is known about the CO₂-concentrating mechanisms and the properties of the enzymes involved in brown algae. Here, we obtained soluble recombinant PEPCKs of five brown algae and carried out biochemical analyses. The five PEPCKs were ATP-dependent and displayed similar or higher specific activities comp

SUBMITTER: Jin JQ 

PROVIDER: S-EPMC12547629 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature