Sandwich immunoassay for evaluation of serum Kita-Kyushu Lung Cancer Antigen-1 as a potential diagnostic biomarker for gastric cancer.

ABSTRACT:

Background

Kita-Kyushu Lung Cancer Antigen-1 (KK-LC-1) has been proved to be highly expressed in gastric cancer. Nevertheless, no studies have yet investigated the expression levels of KK-LC-1 in the serum of patients with gastric cancer, its diagnostic potential, or its association with immune infiltration.

Methods

Designed Ankyrin Repeat Proteins (DARPins) that specifically target KK-LC-1 were identified through phage display screening. A method for quantifying KK-LC-1 concentration utilizing these DARPins was developed based on the sandwich immunoassay technique, and this method was employed to assess the expression levels of KK-LC-1 in serum samples from both gastric cancer patients and healthy donors.

Results

We found that compared to healthy donors, serum levels

SUBMITTER: Chen T 

PROVIDER: S-EPMC12547711 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature