Amyloidogenic Growth Observation of Stem Bromelain via Atomic Force Microscopy.

ABSTRACT: In this paper, we report on the amyloidogenic fibril formation of stem bromelain (SB) by using atomic force microscopy (AFM). Stem bromelain (SB), a proteolytic enzyme, is widely used in industries and medicine, making it essential to understand the factors affecting aggregation. Amyloid formation entails the assembly of proteins into highly ordered, β-sheet-rich fibrillar structures; yet while heating is a recognized trigger for SB fibrillation, the extent of continued fibril growth at room temperature incubation and its nanoscopic morphological observation remain unexplored. Here, SB was heated in pH 10.8 borate buffer at 65 °C for 10 h, then incubated at room temperature for 1, 3, and 7 days, respectively. A time-course imaging directly visualized the morphological progression from smal

SUBMITTER: Lugo MC 

PROVIDER: S-EPMC12547772 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature