Evaluation of urinary cfDNA workflows for the molecular profiling of malignant disease.

ABSTRACT: Urinary cell-free DNA (ucfDNA) is a promising liquid biopsy analyte obtained by a non-invasive sampling method, particularly valuable in urological cancers due to the proximity of biofluids to the tumor site. However, extremely high fragmentation and low abundance of ucfDNA pose significant analytical challenges. Improper handling and storage can severely affect ucfDNA quality, necessitating optimized pre-analytical workflows for clinical applications. We evaluated multiple pre-analytical workflows, including urine stabilization approaches against unstabilized urine samples. Our results demonstrated that without stabilization, ucfDNA degraded rapidly and minimal quantities remaining after 72 h. In contrast, urine stabilization preserved ucfDNA integrity, enabling successful downstream anal

SUBMITTER: Eberhard A 

PROVIDER: S-EPMC12547846 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature