Detection of dedifferentiated stem cells in the <i>Drosophila</i> testis.

ABSTRACT: Dedifferentiation is the process by which partially or terminally differentiated cells revert to a stem cell state, a conserved mechanism that supports stem cell maintenance across various systems. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) have been identified using a lineage-tracing method that relies on the lineage-specific promoter of the differentiation factor bam. However, the specificity and sensitivity of this method have never been thoroughly evaluated. Here, we estimate "false-positive" and "false-negative" marking rates of the bam-dependent lineage tracing system by combining live imaging with mathematical modeling. Our study not only provides a refined tool to facilitate the investigation of dedifferentiation in the Drosophila GSC system

SUBMITTER: Bener MB 

PROVIDER: S-EPMC12547877 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature